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DCBLD2 / ESDN Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500585

DCBLD2 / ESDN Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

DCBLD2 / ESDN Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human DCBLD2 / ESDN aa 529-775.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 85 kDa

Positive Control

U-2 OS cell lysates.

Conjugation

unconjugated

Reactivity Data

WB
Human
Tested Tested
Mouse
Not Recommended
Rat
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:10,000

Target

Function

Involved in negative regulation of cell growth and wound healing. Located in cell surface. Is integral component of plasma membrane.

Background References

1. Chen J et al. CD146 promotes malignant progression of breast phyllodes tumor through suppressing DCBLD2 degradation and activating the AKT pathway. Cancer Commun (Lond). 2023 Nov

2. Guo L et al. DCBLD2 deletion increases hyperglycemia and induces vascular remodeling by inhibiting insulin receptor recycling in endothelial cells. FEBS J. 2024 Sep

Subcellular Location

Membrane.

UNIPROT

SwissProt: Q96PD2 Human

Synonyms

1700055P21Rik antibody

AW146002 antibody

CLCP1 antibody

coagulation factor V/VIII-homology domains protein 1 antibody

CUB and LCCL domain-containing protein 2 antibody

CUB antibody

CUB, LCCL and coagulation factor V/VIII-homology domains protein 1 antibody

DCBD2_HUMAN antibody

DCBLD2 antibody

Discoidin antibody

Expand

Images

  • Western blot analysis of DCBLD2 / ESDN on U-2 OS cell lysate with Rabbit anti-DCBLD2 / ESDN antibody (<a href="/products/HA500585" style="font-weight: bold;text-decoration: underline;">HA500585</a>) at 1/10,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 85 kDa<br />Observed band size: 110 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500585" style="font-weight: bold;text-decoration: underline;">HA500585</a>) at 1/10,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of DCBLD2 / ESDN on U-2 OS cell lysate with Rabbit anti-DCBLD2 / ESDN antibody (HA500585) at 1/10,000 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 85 kDa
    Observed band size: 110 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500585) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"