GFP Recombinant Rabbit Monoclonal Antibody [SP069-0] (ET1604-26)
Catalog# ET1604-26
Rabbit Monoclonal to GFP
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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Species independent
Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1604-26, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
GFP Recombinant Rabbit Monoclonal Antibody [SP069-0] (ET1604-26)
Immunogen
Recombinant full length protein of aequorea victoria gfp.
Host
Rabbit
Positive Control
K562 cell lysates transfected with GFP.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SP069-0
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:10,000
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IF-Cell
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1:500-1:1,000
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IF-Tissue
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1:500-1:1,000
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IHC-P
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1:1,000-1:5,000
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IP
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2-5 µg/ml.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 27 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
GFP
SYNONYMS
GFP antibody;Green fluorescent protein antibody;yfp antibody
FUNCTION
The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.
CITATIONS
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Lijuan Chen,Yiguo Hu
Targeting glutaminase1 and synergizing with clinical drugs achieved more promising antitumor activity on multiple myeloma
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WB
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mouse
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Citation
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