CD276 Recombinant Rabbit Monoclonal Antibody [PD00-36] (HA721245)
Catalog# HA721245
Rabbit Monoclonal to CD276
Tested for
-
IHC-P
-
FC
-
WB
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of CD276 on different lysates with Rabbit anti-CD276 antibody (HA721245) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: MCF7 cell lysate (20 µg/Lane)
Lane 3: HEK-293 cell lysate (20 µg/Lane)
Lane 4: LoVo cell lysate (20 µg/Lane)
Lane 5: U-2 OS cell lysate (20 µg/Lane)
Lane 6: LNCaP cell lysate (20 µg/Lane)
Lane 7: SH-SY5Y cell lysate (20 µg/Lane)
Lane 8: THP-1 cell lysate (20 µg/Lane)
Lane 9: HCT 116 cell lysate (20 µg/Lane)
Lane 10: Raji cell lysate (negative) (20 µg/Lane)
Predicted band size: 57 kDa
Observed band size: 100 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721245) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: MCF7 cell lysate (20 µg/Lane)
Lane 3: HEK-293 cell lysate (20 µg/Lane)
Lane 4: LoVo cell lysate (20 µg/Lane)
Lane 5: U-2 OS cell lysate (20 µg/Lane)
Lane 6: LNCaP cell lysate (20 µg/Lane)
Lane 7: SH-SY5Y cell lysate (20 µg/Lane)
Lane 8: THP-1 cell lysate (20 µg/Lane)
Lane 9: HCT 116 cell lysate (20 µg/Lane)
Lane 10: Raji cell lysate (negative) (20 µg/Lane)
Predicted band size: 57 kDa
Observed band size: 100 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721245) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.