Loading controls serve as a vital method of verifying the results acquired through western blotting by normalizing the protein levels detected by confirming that protein loading is the same across the gel.
When western blotting, there can be numerous roadblocks to reliable results. Nearly every step of the experiment, from sample preparation to the detection methods, can pose a problem. When normalizing the protein levels detected, loading controls serve as an indispensable method of verifying the results. Loading controls are usually proteins that exhibit high-level, constitutive expression in the cell type or sample.
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