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Human DLD, N-Twin Strep, C-His Tag Protein (HA210504)

Price: Regular price $379.00

Product size

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Catalog# HA210504

Reacts with

  • Human

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Product Details

Product Type

Protein

Product Name

Human DLD, N-Twin Strep, C-His Tag Protein (HA210504)

Predicted molecular mass

55.5 kD

Protein construction description

A DNA sequence encoding the human DLD protein (P09622) (Ala 36-Phe 509) was expressed with a Twin strep tag at the N-terminus and a His tag at the C-terminus.

Accession

P09622

Protein construction

Twin Strep | DLD (36-509) | His

Source

E.coli

Bio Activity

Testing in progress.

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Species

Human

Background

Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of three alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex).The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion.A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A. In monomeric form may have additional moonlighting function as serine protease. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction

Usage Guide

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.

Storage

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20C to -80C It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution

Reconstitute at 250 μg/ml in sterile water.

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