Caspase-3 p12 Recombinant Rabbit Monoclonal Antibody [JE07-51] (HA722235)
Catalog# HA722235
Rabbit Monoclonal to Caspase-3 p12
Tested for
-
WB
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IHC-P
Reacts with
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Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Caspase-3 p12 on different lysates with Rabbit anti-Caspase-3 p12 antibody (HA722235) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 1μM staurosporine for 4 hours cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 treated with 1μM staurosporine for 3 hours cell lysate
Lane 5: C6 cell lysate
Lane 6: C6 treated with 1μM staurosporine for 4 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 32/12 kDa
Observed band size: 32/12 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722235) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 1μM staurosporine for 4 hours cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 treated with 1μM staurosporine for 3 hours cell lysate
Lane 5: C6 cell lysate
Lane 6: C6 treated with 1μM staurosporine for 4 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 32/12 kDa
Observed band size: 32/12 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722235) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.