Mouse IL-1 beta Recombinant Rabbit Monoclonal Antibody - BSA and Azide free (Capture) [PSH04-66] (HA722166)
Catalog# HA722166
Rabbit Monoclonal to Mouse IL-1 beta
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ELISA(Cap)
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Mouse
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722166) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-1β protein (HA210706) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722167, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Mouse IL-1 beta Recombinant Rabbit Monoclonal Antibody - BSA and Azide free (Capture) [PSH04-66] (HA722166)
Immunogen
Recombinant protein within mouse il-1 beta aa 118-269 (p10749).
Host
Rabbit
Positive Control
Recombinant Mouse IL-1β protein (HA210706).
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PSH04-66
APPLICATION DILUTION
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ELISA(Cap)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH04-67] to Mouse IL-1 beta (Detector) (HA722167) and recombinant standard Mouse IL-1 beta (HA210706). The reference range value is 12.3-3000 pg/ml.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 31 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Mouse IL-1 beta
SYNONYMS
Catabolin antibody;H1 antibody;IFN beta inducing factor antibody;IL 1 antibody;IL 1 beta antibody;IL-1 beta antibody;IL1 antibody;IL1 BETA antibody;IL1B antibody;IL1B_HUMAN antibody;IL1F2 antibody;Interleukin 1 beta antibody;Interleukin 1 beta precursor antibody;interleukin 1, beta antibody;Interleukin-1 beta antibody;OAF antibody;Osteoclast activating factor antibody;OTTHUMP00000162031 antibody;Preinterleukin 1 beta antibody;Preinterleukin beta antibody;Pro interleukin 1 beta antibody
SUBCELLULAR LOCATION
Cytoplasm, cytosol, Secreted, Lysosome, extracellular exosome.
FUNCTION
Interleukin-1 beta (IL-1β) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a cytokine protein that in humans is encoded by the IL1B gene. There are two genes for interleukin-1 (IL-1): IL-1 alpha and IL-1 beta (this gene). IL-1β precursor is cleaved by cytosolic caspase 1 (interleukin 1 beta convertase) to form mature IL-1β. IL-1β is a member of the interleukin 1 family of cytokines. This cytokine is produced by activated macrophages, monocytes, and a subset of dentritic cells known as slanDC, as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
CITATIONS
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Zhang Ting,et al
PDCD4 Inhibition Alleviates Neuropathic Pain by regulating Spinal Autophagy and neuroinflammation
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ELISA
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Mouse
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Citation
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