Neuroinflammation Marker Antibody Sampler Kit (HAK21063)
Catalog# HAK21063
Reacts with
-
Human
Western blot analysis of GFAP on different lysates with Rabbit anti-GFAP antibody (ET1601-23) at 1/5,000 dilution.
Lane 1: Human brain tissue lysate
Lane 2: Rat brain tissue lysate
Lane 3: Human cerebellum tissue lysate
Lane 4: Mouse brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 11 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-23) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Lane 1: Human brain tissue lysate
Lane 2: Rat brain tissue lysate
Lane 3: Human cerebellum tissue lysate
Lane 4: Mouse brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 11 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-23) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.