Reactive Exosome Marker Antibody Sampler Kit (HAK21058)
Catalog# HAK21058
Reacts with
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Human
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Mouse
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Rat
Western blot analysis of CD9 on different lysates with Rabbit anti-CD9 antibody (ET1601-9) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (20 µg/Lane)
Lane 3: MCF7 cell lysate (20 µg/Lane)
Lane 4: HCT 116 cell lysate (20 µg/Lane)
Lane 5: HepG2 cell lysate (20 µg/Lane)
Lane 6: SK-MEL-28 cell lysate (20 µg/Lane)
Lane 7: A375 cell lysate (20 µg/Lane)
Lane 8: B16-F1 cell lysate (20 µg/Lane)
Predicted band size: 25 kDa
Observed band size: 23 kDa
Exposure time: 3 minutes 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-9) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (20 µg/Lane)
Lane 3: MCF7 cell lysate (20 µg/Lane)
Lane 4: HCT 116 cell lysate (20 µg/Lane)
Lane 5: HepG2 cell lysate (20 µg/Lane)
Lane 6: SK-MEL-28 cell lysate (20 µg/Lane)
Lane 7: A375 cell lysate (20 µg/Lane)
Lane 8: B16-F1 cell lysate (20 µg/Lane)
Predicted band size: 25 kDa
Observed band size: 23 kDa
Exposure time: 3 minutes 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-9) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.