V5 tag Recombinant Rabbit Monoclonal Antibody [SY30-01] (ET1605-41)
Catalog# ET1605-41
Rabbit Monoclonal to V5 tag
Tested for
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WB
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IF-Cell
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IP
Reacts with
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Species independent
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of V5 tag on different lysates with Rabbit anti-V5 tag antibody (ET1605-41) at 1/5,000 dilution.
Lane 1: 293T cell lysate
Lane 2: 293T transfected with V5-tagged Histone H3.1 (N-terminal) cell lysate
Lane 3: 293T transfected with V5-tagged Nanos homolog 3 (C-terminal) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-41) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T cell lysate
Lane 2: 293T transfected with V5-tagged Histone H3.1 (N-terminal) cell lysate
Lane 3: 293T transfected with V5-tagged Nanos homolog 3 (C-terminal) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-41) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
