Bcl-2 Recombinant Rabbit Monoclonal Antibody [SZ10-03] (ET1603-11)
Catalog# ET1603-11
Rabbit Monoclonal to Bcl-2
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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FC
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Human
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: MCF7 cell lysate (20 µg/Lane)
Lane 4: HL-60 cell lysate (20 µg/Lane)
Lane 5: THP-1 cell lysate (20 µg/Lane)
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-11) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Safety datasheet
Select your chosen country/region
- MSDS_HUABIO.pdf
- MSDS_HUABIO.pdf
- MSDS_ET1603-11_Europe.pdf
- No MSDS Found
COA DOWNLOAD
- No COA Found
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Bcl-2 Recombinant Rabbit Monoclonal Antibody [SZ10-03] (ET1603-11)
Immunogen
Synthetic peptide within human bcl-2 aa 40-90.
Host
Rabbit
Positive Control
HeLa cell lysate, Jurkat cell lysate, MCF7 cell lysate, HL-60 cell lysate, THP-1 cell lysate, A549, MCF-7, SH-SY5Y, human lymph nodes tissue, human kidney tissue, Jurkat.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SZ10-03
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:800
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IHC-P
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1:50-1:4,000
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FC
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1:50-1:100
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 26 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Bcl-2
SYNONYMS
Apoptosis regulator Bcl 2 antibody;Apoptosis regulator Bcl-2 antibody;Apoptosis regulator Bcl2 antibody;AW986256 antibody;B cell CLL/lymphoma 2 antibody;B cell leukemia/lymphoma 2 antibody;Bcl-2 antibody;Bcl2 antibody;BCL2_HUMAN antibody;C430015F12Rik antibody;D630044D05Rik antibody;D830018M01Rik antibody;Leukemia/lymphoma, B-cell, 2 antibody;Oncogene B-cell leukemia 2 antibody;PPP1R50 antibody;Protein phosphatase 1, regulatory subunit 50 antibody;Bcl 2 antibody
SEQUENCE SIMILARITIES
Belongs to the Bcl-2 family.
TISSUE SPECIFICITY
Expressed in a variety of tissues.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity).; Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.; Monoubiquitinated by PRKN, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.
SUBCELLULAR LOCATION
Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm.
FUNCTION
Apoptosis is defined as a set of cascades which, when initiated, programs the cell to undergo lethal changes such as membrane blebbing, mitochondrial break down and DNA fragmentation. Bcl-2 is one among many key regulators of apoptosis, which are essential for proper development, tissue homeostasis, and protection against foreign pathogens. Human Bcl-2 is an anti-apoptotic, membrane-associated oncoprotein that can promote cell survival through protein-protein interactions with other Bcl-2 related family members, such as the death suppressors Bcl-xl, Mcl-1, Bcl-w, and A1 or the death agonists Bax, Bak, Bik, Bad, and Bid. The anti-apoptotic function of Bcl-2 can also be regulated through proteolytic processing and phospho-rylation. Bcl-2 may promote cell survival by interfering with the activation of the cytochrome c/Apaf-1 pathway through stabilization of the mitochondrial membrane. Mutations in the Bcl-2 gene can contribute to cancers where normal physiological cell death mechanisms are compromised by deregulation of the anti-apoptotic influence of Bcl-2.
CITATIONS
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Fan, H., Ren, Z., Xu, C., Wang, H., Wu, Z., Rehman, Z. U., Wu, S., Sun, M. A., & Bao, W.
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Wu Xiaoqi,et al
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Mouse
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He, S., Yu, X., Cui, D., Liu, Y., Yang, S., Zhang, H., Hu, W., & Su, Z.
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Analysis of the roles of the Notch1 signalling pathway in modulating deoxynivalenol cytotoxicity
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Gao, Z., Xu, C., Fan, H., Wang, H., Wu, Z., Wu, S., & Bao, W.
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Pig
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Zhao Q, He W, Liu Z, et al
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IF
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Liangen Shi
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