Cdk4 Recombinant Rabbit Monoclonal Antibody [SD20-42] (ET1612-1)

UNIPROT #

P11802

PROTEIN NAME

Cdk4

SYNONYMS

Cdk 4 antibody;cdk4 antibody;CDK4 protein antibody;CDK4_HUMAN antibody;Cell division kinase 4 antibody;Cell division protein kinase 4 antibody;CMM 3 antibody;CMM3 antibody;Crk3 antibody;Cyclin dependent kinase 4 antibody;Cyclin-dependent kinase 4 antibody;Melanoma cutaneous malignant 3 antibody;MGC14458 antibody;p34 cdk4 antibody;PSK J3 antibody;PSK-J3 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.

SUBCELLULAR LOCATION

Nucleus, Nucleus membrane, Cytoplasm.

FUNCTION

Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression . Another family of proteins, Cdk inhibitors, also plays a role in regulating the cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2-Cdk8, PCTAIRE-1-PCTAIRE-3, PITALRE and PITSLRE. Cdk4, in complex with D-type cyclins, is thought to regulate cell growth during the G1 phase of the cell cycle. This association with a D-type cyclin upregulates Cdk4 activity, whereas binding to the Cdk inhibitor p16 downregulates Cdk4 activity. Activation of the Cdk4-cyclin complexes requires phosphorylation on a single threonyl residue of Cdk4, catalyzed by a Cdk-activating protein (CAK).