Nanog Recombinant Rabbit Monoclonal Antibody [SC05-70] (ET1610-2)

UNIPROT #

Q9H9S0

PROTEIN NAME

Nanog

SYNONYMS

Embryonic stem cell specific homeobox protein (Nanog) antibody;ENK antibody;FLJ12581 antibody;hNanog antibody;Homeobox protein NANOG antibody;Homeobox transcription factor Nanog antibody;homeobox transcription factor Nanog-delta 48 antibody;NANOG antibody;Nanog homeobox antibody;NANOG_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the Nanog homeobox family.

TISSUE SPECIFICITY

Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.

DEVELOPMENTAL STAGE

Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Nanog (from “Tir Na Nog,” the mythologic Celtic land of the ever young) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines and absent from differentiated cells. Human Nanog protein shares 52% overall amino acid identity with the mouse protein and 85% identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst. High levels of human Nanog expression have been detected by Northern analysis in the undifferentiated NTERA-2 cl.D1 embryonal carcinoma cell line.