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MCL1 Recombinant Rabbit Monoclonal Antibody [SI16-04] (ET1606-14)

Price: Regular price $385.00

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Catalog# ET1606-14

Rabbit Monoclonal to MCL1

Tested for

  • WB

  • IF-Tissue

  • IHC-P

  • IP

Reacts with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
This product has been cited in peer reviewed publications, see list HERE

Safety datasheet

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Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

MCL1 Recombinant Rabbit Monoclonal Antibody [SI16-04] (ET1606-14)

Immunogen

Synthetic peptide within human mcl1 aa 101-150 / 350.

Host

Rabbit

Positive Control

Raji cell lysate, Ramos cell lysate, MCF7 cell lysate, SK-OV-3 cell lysate, mouse heart tissue lysates, Hela, HepG2, BT-20, human breast tissue, human kidney tissue, human pancreas tissue, mouse kidney tissue, Jurkat.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SI16-04

RRID

AB_3069723

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • IP

  • Use at an assay dependent concentration.

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 37 kDa

Isotype

IgG

TARGET

UNIPROT #

Q07820

PROTEIN NAME

MCL1

SYNONYMS

Bcl 2 related protein EAT/mcl1 antibody;Bcl-2-like protein 3 antibody;Bcl-2-related protein EAT/mcl1 antibody;BCL2 related antibody;Bcl2-L-3 antibody;BCL2L3 antibody;EAT antibody;Induced myeloid leukemia cell differentiation protein Mcl 1 antibody;Induced myeloid leukemia cell differentiation protein Mcl-1 antibody;MCL 1 antibody;MCL1 antibody;MCL1-ES antibody;mcl1/EAT antibody;MCL1_HUMAN antibody;MCL1L antibody;MCL1S antibody;MGC104264 antibody;MGC1839 antibody;Myeloid Cell Leukemia 1 antibody;Myeloid cell leukemia ES antibody;Myeloid cell leukemia sequence 1 antibody;Myeloid cell leukemia sequence 1 BCL2 related antibody;Myeloid cell leukemia sequence 1 isoform 1 antibody;OTTHUMP00000032794 antibody;OTTHUMP00000032795 antibody;TM antibody

SEQUENCE SIMILARITIES

Belongs to the Bcl-2 family.

POST-TRANSLATIONAL MODIFICATION

Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.; Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.; Phosphorylated on Ser-159, by GSK3, in response to IL3/interleukin-3 withdrawal. Phosphorylation at Ser-159 induces ubiquitination and proteasomal degradation, abrogating the anti-apoptotic activity. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.; Ubiquitinated. Ubiquitination is induced by phosphorylation at Ser-159.

SUBCELLULAR LOCATION

Membrane, Cytoplasm, Mitochondrion, Nucleus.

FUNCTION

B-cell CLL/lymphoma 2 (Bcl-2) blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Myeloid cell leukemia 1 (Mcl-1) shares sequence homology with Bcl-2 and further resembles Bcl-2 in that its expression promotes cell viability. p53 and Mcl-1 demonstrate opposing effects on mitochondrial apoptosis by mediating Bcl-2 antagonist killer (Bak) activity. Mcl-1 is an important and specific regulator that is necessary for the homeostasis of early hematopoietic progenitors. Glycogen synthase kinase 3 (GSK3) controls Mcl-1 stability, which has an effect on the regulation of apoptosis by growth factors, PI 3-kinase and AKT. Mice with a deficiency of the Mcl-1 protein show a significant reduction in B and T lymphocytes similar to the effects observed in IL-7- or IL-7R-deficient mice.

CITATIONS

  • Liu, P., Qi, G., Gu, S., Dong, H., Liu, C., & Yang, H.

    Single-cell transcriptomics and network pharmacology reveal therapeutic targets of Jianpi Yiqi Bugan Yishen decoction in immune cell subsets of children with myasthenia gravis

  • Chen, Y., Zhang, W., Bai, X., & Liu, Y.

    Targeting the transcriptional activity of STAT3 by a novel fusion protein

  • Fan Chengcheng,et al

    Natural Product-Inspired Discovery of Naphthoquinone-Furo-Piperidine Derivatives as Novel STAT3 Inhibitors for the Treatment of Triple-Negative Breast Cancer

  • Methylcrotonyl-CoA carboxylase subunit 1 (MCCA) regulates multidrug resistance in multiple myeloma

  • Intracellular angiopoietin-1 promotes TKI-resistance via activation of JAK/STAT5 pathway in chronic myeloid leukemia

  • Yingying Sun , Le Xu , Yi Li , Shunze Jia , Gang Wang , Xufeng Cen , Yuyan Xu , Zhongkai Cao , Jingjing Wang , Ning Shen , Lidan Hu , Jin Zhang , Jianhua Mao , Hongguang Xia , Zhihong Liu , Xudong Fu

    Mitophagy defect mediates the aging-associated hallmarks in Hutchinson-Gilford progeria syndrom

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