Topoisomerase II alpha Recombinant Rabbit Monoclonal Antibody [SY27-00] (ET1607-59)
Catalog# ET1607-59
Rabbit Monoclonal to Topoisomerase Ⅱ alpha
Tested for
-
WB
-
IHC-P
-
IP
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Topoisomerase II alpha on different lysates with Rabbit anti-Topoisomerase II alpha antibody (ET1607-59) at 1/1,000 dilution.
Lane 1: Rat testis tissue lysate (40 µg/Lane)
Lane 2: Mouse testis tissue lysate (40 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: HT-29 cell lysate (20 µg/Lane)
Lane 5: Jurkat cell lysate (20 µg/Lane)
Predicted band size: 174 kDa
Observed band size: 174 kDa
Exposure time: Lane 1-4: 3 minutes; Lane 5: 18 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-59) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Rat testis tissue lysate (40 µg/Lane)
Lane 2: Mouse testis tissue lysate (40 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: HT-29 cell lysate (20 µg/Lane)
Lane 5: Jurkat cell lysate (20 µg/Lane)
Predicted band size: 174 kDa
Observed band size: 174 kDa
Exposure time: Lane 1-4: 3 minutes; Lane 5: 18 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-59) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.