HER2 / ErbB2 Rabbit Polyclonal Antibody (ER0106)
Catalog# ER0106
Rabbit Polyclonal to HER2 / ErbB2
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of HER2 / ErbB2 on different lysates with Rabbit anti-HER2 / ErbB2 antibody (ER0106) at 1/1,000 dilution.
Lane 1: SK-Br-3 cell lysate
Lane 2: SKOV-3 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 138 kDa
Observed band size: 180 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER0106) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: SK-Br-3 cell lysate
Lane 2: SKOV-3 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 138 kDa
Observed band size: 180 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER0106) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
