Phospho-EGFR (Y845) Recombinant Rabbit Monoclonal Antibody [JE46-77] (HA721488)
Catalog# HA721488
Rabbit Monoclonal to Phospho-EGFR (Y845)
Tested for
-
WB
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Phospho-EGFR (Y845) on different lysates with Rabbit anti-Phospho-EGFR (Y845) antibody (HA721488) at 1/1,000 dilution.
Lane 1: A431 whole cell lysate
Lane 2: A431 treated with 100ng/mL EGF for 10 minutes whole cell lysate
Lane 3: A431 whole cell lysate treated with λpp for 1 hour
Lane 4: A431 treated with 100ng/mL EGF for 10 minutes whole cell lysate treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 134 kDa
Observed band size: 180/75 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721488) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: A431 whole cell lysate
Lane 2: A431 treated with 100ng/mL EGF for 10 minutes whole cell lysate
Lane 3: A431 whole cell lysate treated with λpp for 1 hour
Lane 4: A431 treated with 100ng/mL EGF for 10 minutes whole cell lysate treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 134 kDa
Observed band size: 180/75 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721488) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.