FGF2 Recombinant Rabbit Monoclonal Antibody [JM28-10] (ET1703-18)
Catalog# ET1703-18
Rabbit Monoclonal to FGF2
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WB
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IP
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FC
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IHC-P
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Human
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Mouse
Lane 1: K-562 cell lysate (20 µg/Lane)
Lane 2: U-87 MG cell lysate (20 µg/Lane)
Lane 3: HeLa cell lysate (20 µg/Lane)
Lane 4: 293T cell lysate (20 µg/Lane)
Predicted band size: 31 kDa
Observed band size: 18/22/24 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-18) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
FGF2 Recombinant Rabbit Monoclonal Antibody [JM28-10] (ET1703-18)
Immunogen
Synthetic peptide within human fgf2 aa 121-170 / 288.
Host
Rabbit
Positive Control
K-562 cell lysate, U-87 MG cell lysate, HeLa cell lysate, 293T cell lysate, human breast tissue, human prostate tissue, mouse trachea tissue, Jurkat.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM28-10
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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IP
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1-2μg/sample
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FC
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1:50-1:100
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IHC-P
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1:200-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 31 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
FGF2
SYNONYMS
Basic fibroblast growth factor antibody;Basic fibroblast growth factor bFGF antibody;BFGF antibody;FGF 2 antibody;FGF B antibody;FGF-2 antibody;Fgf2 antibody;FGF2 basic antibody;FGF2_HUMAN antibody;FGFB antibody;Fibroblast growth factor 2 (basic) antibody;Fibroblast growth factor 2 antibody;Fibroblast growth factor, basic antibody;HBGF 2 antibody;HBGF-2 antibody;HBGF2 antibody;HBGH 2 antibody;HBGH2 antibody;Heparin binding growth factor 2 precursor antibody;Heparin-binding growth factor 2 antibody;Prostatropin antibody
SEQUENCE SIMILARITIES
Belongs to the heparin-binding growth factors family.
TISSUE SPECIFICITY
Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.; Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
SUBCELLULAR LOCATION
Secreted, Nucleus.
FUNCTION
FGF2, also known as basic fibroblast growth factor (bFGF) and FGF-β, is a growth factor and signaling protein encoded by the FGF2 gene. It binds to and exerts effects via specific fibroblast growth factor receptor (FGFR) proteins, themselves a family of closely related molecules. Fibroblast growth factor protein was first purified in 1975; soon thereafter three variants were isolated: 'basic FGF' (FGF2); Heparin-binding growth factor-2; and Endothelial cell growth factor-2. Gene sequencing revealed that this group is the same FGF2 protein and is a member of a family of FGF proteins. Like other FGF family members, basic fibroblast growth factor possess broad mitogenic and cell survival activities, and is involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. In normal tissue, bFGF is present in basement membranes and in the subendothelial extracellular matrix of blood vessels. It stays membrane-bound as long as there is no signal peptide.
CITATIONS
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Ma Shilong,et al
Gelatin‑sodium alginate composite hydrogel doped with black phosphorus@ZnO heterojunction for cutaneous wound healing with antibacterial, immunomodulatory, and angiogenic properties
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WB
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Human
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Citation
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