Phospho-PDGFR beta (Y740) Recombinant Rabbit Monoclonal Antibody [SN0646] (ET1611-29)

UNIPROT #

P09619

PROTEIN NAME

Phospho-PDGFR beta (Y740)

SYNONYMS

Beta platelet derived growth factor receptor antibody;Beta-type platelet-derived growth factor receptor antibody;CD 140B antibody;CD140 antigen-like family member B antibody;CD140b antibody;CD140b antigen antibody;IBGC4 antibody;IMF1 antibody;JTK12 antibody;OTTHUMP00000160528 antibody;PDGF R beta antibody;PDGF-R-beta antibody;PDGFR 1 antibody;PDGFR antibody;PDGFR beta antibody;PDGFR1 antibody;PDGFRB antibody;PGFRB_HUMAN antibody;Platelet derived growth factor receptor 1 antibody;Platelet derived growth factor receptor beta antibody;Platelet derived growth factor receptor beta polypeptide antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

TISSUE SPECIFICITY

Weakly expressed in glomerular mesangial cells and interstitial cells. Up-regulated in areas of renal fibrosis. In mice with unilateral ureteral obstruction, increased expression in interstitial cells at day 4 and expression is markedly elevated at day 7 and is maximal at day 14.

POST-TRANSLATIONAL MODIFICATION

Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-578, and to a lesser degree, Tyr-580 is important for interaction with SRC. Phosphorylation at Tyr-715 is important for interaction with GRB2. Phosphorylation at Tyr-739 and Tyr-750 is important for interaction with PIK3R1. Phosphorylation at Tyr-750 is important for interaction with NCK1. Phosphorylation at Tyr-770 and Tyr-856 is important for interaction with RASA1/GAP. Phosphorylation at Tyr-856 is important for efficient phosphorylation of PLCG1 and PTPN11, resulting in increased phosphorylation of AKT1, MAPK1/ERK2 and/or MAPK3/ERK1, PDCD6IP/ALIX and STAM, and in increased cell proliferation. Phosphorylation at Tyr-1008 is important for interaction with PTPN11. Phosphorylation at Tyr-1008 and Tyr-1020 is important for interaction with PLCG1. Dephosphorylated by PTPRJ at Tyr-750, Tyr-856, Tyr-1008 and Tyr-1020 (By similarity). Dephosphorylated by PTPN2 at Tyr-578 and Tyr-1020.; N-glycosylated.; Ubiquitinated. After autophosphorylation, the receptor is polyubiquitinated, leading to its degradation.

SUBCELLULAR LOCATION

Cell membrane, Cytoplasmic vesicle.

FUNCTION

Platelet Derived Growth Factor (PDGF) is a mitogen for mesenchyme- and glia-derived cells. PDGF consists of two chains, A and B, which dimerize to form functionally distinct isoforms, PDGF-AA, PDGF-AB, and PDGF-BB. These three isoforms bind with different affinities to two receptor types, a and b, which are endowed with protein tyrosine kinase domains and undergo either homo- or heterodimerization as a consequence of ligand binding. Ligand stimulation of PDGFR-b leads to autophosphorylation at Tyr-857, which is the major autophosphorylation site, and Tyr-751, which is the major in vitro phosphorylation site. Autophosphorylation of Tyr-751, which lies in the kinase insert region, is required for binding of phosphatidylinositol-3 kinase to the receptor. These auto-phosphorylation events largely contribute to signal transduction through the PDGF receptor. Mutations in PDGFRB are mainly associated with the clonal eosinophilia class of malignancies.