Phospho-Smad2 (S250) Recombinant Rabbit Monoclonal Antibody [SD207-1] (ET1612-32)

UNIPROT #

Q15796

PROTEIN NAME

Phospho-Smad2 (S250)

SYNONYMS

Drosophila, homolog of, MADR2 antibody;hMAD-2 antibody;HsMAD2 antibody;JV18 antibody;JV18-1 antibody;JV181 antibody;MAD antibody;MAD homolog 2 antibody;MAD Related Protein 2 antibody;Mad-related protein 2 antibody;MADH2 antibody;MADR2 antibody;MGC22139 antibody;MGC34440 antibody;Mother against DPP homolog 2 antibody;Mothers against decapentaplegic homolog 2 antibody;Mothers against decapentaplegic, Drosophila, homolog of, 2 antibody;Mothers against DPP homolog 2 antibody;OTTHUMP00000163489 antibody;Sma and Mad related protein 2 antibody;Sma- and Mad-related protein 2 MAD antibody;SMAD 2 antibody;SMAD family member 2 antibody;SMAD, mothers against DPP homolog 2 antibody;SMAD2 antibody;SMAD2_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the dwarfin/SMAD family.

TISSUE SPECIFICITY

Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. TGF-beta-induced Ser-465/467 phosphorylation declines progressively in a KMT5A-dependent manner. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. Phosphorylated by PDPK1.; In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation. Monoubiquitinated, leading to prevent DNA-binding (By similarity). Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes. Ubiquitinated by RNF111, leading to its degradation: only SMAD2 proteins that are 'in use' are targeted by RNF111, RNF111 playing a key role in activating SMAD2 and regulating its turnover (By similarity).; Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to Activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.