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active+pro Caspase-3 Recombinant Rabbit Monoclonal Antibody [SU38-04] (ET1608-64)

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Catalog# ET1608-64

Rabbit Monoclonal to active+pro Caspase-3

Tested for

  • WB

  • IHC-P

  • IP

Reacts with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
This product has been cited in peer reviewed publications, see list HERE

Safety datasheet

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Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

active+pro Caspase-3 Recombinant Rabbit Monoclonal Antibody [SU38-04] (ET1608-64)

Immunogen

Recombinant protein within human caspase-3 aa 9-175 / 277.

Host

Rabbit

Positive Control

HeLa cell lysate, HeLa treated with 1μM staurosporine for 4 hours cell lysate, HeLa treated with 3μM staurosporine for 4 hours cell lysate, C6 cell lysate, C6 treated with 1μM staurosporine for 4 hours cell lysate, C6 treated with 3μM staurosporine for 4 hours cell lysate, U-87 MG cell lysate, MDA-MB-231 cell lysate, NIH/3T3 cell lysate, Jurkat cell lysate, Jurkat treated with 1μM staurosporine for 3 hours cell lysate, human lung carcinoma tissue, human spleen tissue, human liver tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SU38-04

RRID

AB_3069820

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:1,000

  • IP

  • Use at an assay dependent concentration.

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 32/17 kDa

Isotype

IgG

TARGET

UNIPROT #

P42574

PROTEIN NAME

active+pro Caspase-3

SYNONYMS

Caspase3;A830040C14Rik antibody;Apopain antibody;CASP 3 antibody;CASP-3 antibody;CASP3 antibody;CASP3_HUMAN antibody;Casp3a antibody;Caspase 3 antibody;Caspase 3, apoptosis-related cysteine peptidase antibody;Caspase 3, apoptosis-related cysteine protease antibody;Caspase 3, apoptosis-related cysteine protease a antibody;Caspase-3 subunit p12 antibody;Caspase3 antibody;CC3 antibody;CPP 32 antibody;CPP-32 antibody;CPP32 antibody;CPP32B antibody;Cysteine protease CPP32 antibody;EC 3.4.22.56 antibody;ICE3 antibody;LICE antibody;mldy antibody;OTTHUMP00000165052 antibody;OTTHUMP00000165053 antibody;OTTHUMP00000165054 antibody;PARP cleavage protease antibody;Procaspase3 antibody;Protein Yama antibody;SCA 1 antibody;SCA-1 antibody;SCA1 antibody;SREBP cleavage activity 1 antibody;Yama antibody;Yama protein antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase C14A family.

TISSUE SPECIFICITY

Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

POST-TRANSLATIONAL MODIFICATION

Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.; S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9. It is encoded by the CASP3 gene. CASP3 orthologs have been identified in numerous mammals for which complete genome data are available. Unique orthologs are also present in birds, lizards, lissamphibians, and teleosts. Caspase-3 shares many of the typical characteristics common to all currently-known caspases. For example, its active site contains a cysteine residue (Cys-163) and histidine residue (His-121) that stabilize the peptide bond cleavage of a protein sequence to the carboxy-terminal side of an aspartic acid when it is part of a particular 4-amino acid sequence. This specificity allows caspases to be incredibly selective, with a 20,000-fold preference for aspartic acid over glutamic acid. A key feature of caspases in the cell is that they are present as zymogens, termed procaspases, which are inactive until a biochemical change causes their activation. Each procaspase has an N-terminal large subunit of about 20 kDa followed by a smaller subunit of about 10 kDa, called p20 and p10, respectively.

CITATIONS

  • Wu, R., Xi, Z., Liu, M., Ren, H., Dai, R., Jiang, X., Nik Nabil, W. N., Wang, Y., Feng, J., Chai, Q., Dong, Q., & Xu, H.

    Pulsatilla Decoction and its bioactive component β-peltatin induce G2/M cell cycle arrest and apoptosis in pancreatic cancer

  • Xia, Z., Li, M., Hu, M., Lin, Y., Atteh, L. L., Fu, W., Gao, L., Bai, M., Huang, C., Yue, P., Liu, Y., & Meng, W.

    Phosphoproteomics reveals that cinobufotalin promotes intrahepatic cholangiocarcinoma cell apoptosis by activating the ATM/CHK2/p53 signaling pathway

  • Fan, H., Ren, Z., Xu, C., Wang, H., Wu, Z., Rehman, Z. U., Wu, S., Sun, M. A., & Bao, W.

    Chromatin Accessibility and Transcriptomic Alterations in Murine Ovarian Granulosa Cells upon Deoxynivalenol Exposure

  • Chaetocin exhibits anticancer effects in esophageal squamous cell carcinoma via activation of Hippo pathway

  • Jiang Huangyu,et al

    Evodiamine Inhibits the Progression of Esophageal Aquamous Cell Carcinoma via Modulating PI3K/AKT/mTOR Pathway

  • Yuan Chen,et al

    Chidan Tuihuang Granule Modulates Gut Microbiota to Influence NOD1/RIPK2 Pathway in Cholestatic Liver Injury Recovery

  • Jiapeng Huang,et al

    Dual-tDCS Ameliorates Cerebral Injury and Promotes Motor Function Recovery via cGAS-STING Signaling Pathway in a Rat Model of Ischemic Stroke

  • Wang, Y., Luan, S., Yuan, Z., Lin, C., Fan, S., Wang, S., Ma, C., & Wu, S.

    Therapeutic effect of platelet-rich plasma on glucocorticoid-induced rat bone marrow mesenchymal stem cells in vitro

  • Fan, H., Wang, S., Wang, H., Sun, M., Wu, S., & Bao, W.

    Melatonin Ameliorates the Toxicity Induced by Deoxynivalenol in Murine Ovary Granulosa Cells by Antioxidative and Anti-Inflammatory Effects. Antioxidants (Basel, Switzerland), 10(7), 1045.

  • Shi, D., Shan, Y., Zhu, X., Wang, H., Wu, S., Wu, Z., & Bao, W.

    Histone Methyltransferase MLL1 Mediates Oxidative Stress and Apoptosis upon Deoxynivalenol Exposure in the Intestinal Porcine Epithelial Cells

  • Reduced HMGB1 expression contributed to lapatinib-induced cutaneous injury

  • Crizotinib induces pulmonary toxicity by blocking autophagy flux in alveolar epithelial cells

  • Chen Chuyi,et al

    Cardamonin attenuates iron overload-induced osteoblast oxidative stress through the HIF-1α/ROS pathway

  • Decreased HMGB1 expression contributed to cutaneous toxicity caused by lapatinib

  • Huang Yating,et al

    Resistance exercise preconditioning prevents disuse muscle atrophy by inhibiting apoptosis and protein degradation via SESN2 in C57BL/6J mice

  • Exploring the mechanism of an active ingredient of ginger, dihydrocapsaicin, on triple negative breast cancer based on network pharmacology and in vitro experiments

  • Ren, Q., Wang, B., Guo, F., Huang, R., Tan, Z., Ma, L., & Fu, P.

    Natural Flavonoid Pectolinarigenin Alleviated Hyperuricemic Nephropathy via Suppressing TGFβ/SMAD3 and JAK2/STAT3 Signaling Pathways

  • Li Yantao,et al

    Bovine lactoferrin inhibits inflammatory response and apoptosis in lipopolysaccharide-induced acute lung injury by targeting the PPAR-γ pathway

  • Wang, A., Li, L., Li, M., Wang, S., & Wang, C.

    Case Report: 7-Ethyl-10-Hydroxycamptothecin, a DNA Topoisomerase I Inhibitor, Performs BRD4 Inhibitory Activity and Inhibits Human Leukemic Cell Growth. Frontiers in pharmacology, 12, 664176.

  • Wang, Y., Luan, S., Yuan, Z., Wang, S., Fan, S., Ma, C., & Wu, S.

    The Combined Use of Platelet-Rich Plasma Clot Releasate and Allogeneic Human Umbilical Cord Mesenchymal Stem Cells Rescue Glucocorticoid-Induced Osteonecrosis of the Femoral Head

  • Yin, P., Li, D., Zhao, Q., Cai, M., Wu, Z., Shi, Y., & Su, L.

    Gsα deficiency facilitates cardiac remodeling via CREB/ Bmp10-mediated signaling

  • Bisdemethoxycurcumin alleviates vandetanib-induced cutaneous toxicity in vivo and in vitro through autophagy activation

  • Zhao, X. Q., Wan, H. Y., He, S. Y., Qin, H. J., Yu, B., & Jiang, N.

    Vitamin D Receptor Genetic Polymorphisms Associate With a Decreased Susceptibility to Extremity Osteomyelitis Partly by Inhibiting Macrophage Apoptosis Through Inhibition of Excessive ROS Production via VDR-Bmi1 Signaling

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