VCAM1 Recombinant Rabbit Monoclonal Antibody [SA05-04] (ET1601-18)
Catalog# ET1601-18
Rabbit Monoclonal to VCAM1
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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FC
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Human
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Mouse
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Rat
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Zebrafish
Lane 1: C2C12 cell lysate (20 µg/Lane)
Lane 2: 3T3-L1 cell lysate (20 µg/Lane)
Lane 3: Mouse spleen tissue lysate (20 µg/Lane)
Lane 4: Rat spleen tissue lysate (20 µg/Lane)
Predicted band size: 81 kDa
Observed band size: 100 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-18) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
VCAM1 Recombinant Rabbit Monoclonal Antibody [SA05-04] (ET1601-18)
Immunogen
Synthetic peptide within human vcam1 aa 690-739 / 739.
Host
Rabbit
Positive Control
C2C12 cell lysate, 3T3-L1 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, NIH/3T3 cell lysate, human spleen tissue, C2C12 cell lysate, C2C12.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SA05-04
RRID
APPLICATION DILUTION
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WB
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1:5,000-1:10,000
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IF-Cell
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1:1,000
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IF-Tissue
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1:100
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IHC-P
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1:200
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IP
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1-2μg/sample
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 81 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
VCAM1
SYNONYMS
CD106 antibody;CD106 Antigen antibody;INCAM 100 antibody;INCAM-100 antibody;L1CAM antibody;MGC99561 antibody;V-CAM 1 antibody;Vascular Cell Adhesion Molecule 1 antibody;Vascular cell adhesion protein 1 antibody;VCAM 1 antibody;VCAM-1 antibody;VCAM1 antibody;VCAM1_HUMAN antibody
TISSUE SPECIFICITY
Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.
POST-TRANSLATIONAL MODIFICATION
Sialoglycoprotein.
SUBCELLULAR LOCATION
Membrane
FUNCTION
Vascular cell adhesion protein 1 also known as vascular cell adhesion molecule 1 (VCAM-1) or cluster of differentiation 106 (CD106) is a protein that in humans is encoded by the VCAM1 gene. VCAM-1 functions as a cell adhesion molecule. The VCAM-1 protein mediates the adhesion of lymphocytes, monocytes, eosinophils, and basophils to vascular endothelium. It also functions in leukocyte-endothelial cell signal transduction, and it may play a role in the development of atherosclerosis and rheumatoid arthritis.
CITATIONS
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Huang, F., Liu, X., Xu, S., Hu, S., Wang, S., Shi, D., Wang, K., Wang, Z., Lin, Q., Li, S., Zhao, S., Jin, K., Wang, C., Chen, L., & Wang, F.
Prevotella histicola Mitigated Estrogen Deficiency-Induced Depression via Gut Microbiota-Dependent Modulation of Inflammation in Ovariectomized Mice
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WB
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Mouse
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Citation
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Yafei Wu
Fusobacterium nucleatum stimulates monocyte adhesion to and transmigration through endothelial cells
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WB
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HUVEC cell
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Citation
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Daisong Li,et al
Integrated metabolomics and network pharmacology to reveal the mechanisms of Shexiang Baoxin pill against atherosclerosis
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WB
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Mouse
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Citation
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Jingyi Xu,et al
Obtusin ameliorates diabetic retinopathy by inhibiting oxidative stress and inflammation
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WB
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Mouse
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Citation
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Oral administration of MnCl2 attenuated hyperlipidemia-related cardiac remodeling in ApoE−/− mice
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IHC
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Mouse
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Citation
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He Jia,et al
Unveiling the Impact of Hemodynamics on Endothelial Inflammation-Mediated Hepatocellular Carcinoma Metastasis Using a Biomimetic Vascular Flow Model
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WB
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IF
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Human
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Citation
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Sox10 escalates vascular inflammation by mediating vascular smooth muscle cell transdifferentiation and pyroptosis in neointimal hyperplasia
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WB
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Mouse
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Citation
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Peng Sha,et al
Dipsacoside B ameliorates cognitive impairment in sepsis-associated encephalopathy by reducing Th17 cell infiltration and neuroinflammation
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WB
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Mouse
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Citation
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Han Sun,et al
Isoechinulin B, a natural product from Antarctic fungus, attenuates acute liver injury by inhibiting excessive cell adhesion
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IF
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Human
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Citation
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