Bcl-2 Mouse Monoclonal Antibody [9F3] (EM1701-83)
Catalog# EM1701-83
Mouse Monoclonal to Bcl-2
Tested for
-
WB
-
IHC-P
-
FC
Reacts with
-
Human
Western blot analysis of Bcl-2 on different lysates with Mouse anti-Bcl-2 antibody (EM1701-83) at 1/1,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: HL-60 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-83) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: THP-1 cell lysate
Lane 2: HL-60 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-83) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.