ATG4A Recombinant Rabbit Monoclonal Antibody [JE32-31] (HA721010)
Catalog# HA721010
Rabbit Monoclonal to ATG4A
Tested for
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WB
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IHC-P
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FC
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IF-Cell
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of ATG4A on different lysates with Rabbit anti-ATG4A antibody (HA721010) at 1/500 dilution.
Lane 1: K562 cell lysate, 10 µg/Lane
Lane 2: Jurkat cell lysate, 10 µg/Lane
Lane 3: Mouse kidney tissue lysate, 20 µg/Lane
Lane 4: Rat kidney tissue lysate, 20 µg/Lane
Predicted band size: 45 kDa
Observed band size: 41 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721010) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Lane 1: K562 cell lysate, 10 µg/Lane
Lane 2: Jurkat cell lysate, 10 µg/Lane
Lane 3: Mouse kidney tissue lysate, 20 µg/Lane
Lane 4: Rat kidney tissue lysate, 20 µg/Lane
Predicted band size: 45 kDa
Observed band size: 41 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721010) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.