Ras Recombinant Rabbit Monoclonal Antibody [SA39-05] (ET1601-16)
Catalog# ET1601-16
Rabbit Monoclonal to Ras
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WB
-
IF-Cell
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FC
-
IP
-
Human
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Mouse
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Rat
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Zebrafish
Lane 1: 293T cell lysate (10 µg/Lane)
Lane 2: MCF7 cell lysate (10 µg/Lane)
Lane 3: NIH/3T3 cell lysate (10 µg/Lane)
Lane 4: Neuro-2a cell lysate (10 µg/Lane)
Lane 5: PC-12 cell lysate (10 µg/Lane)
Lane 6: Mouse brain tissue lysate (20 µg/Lane)
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-16) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Ras Recombinant Rabbit Monoclonal Antibody [SA39-05] (ET1601-16)
Immunogen
Synthetic peptide within human ras aa 7-56 / 189.
Host
Rabbit
Positive Control
293T cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, MCF7, NIH/3T3, PC-12.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SA39-05
RRID
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
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IF-Cell
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1:100
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FC
-
1:1,000
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IP
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1-2μg/sample
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 21 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Ras
SYNONYMS
C-BAS/HAS antibody;c-H-ras antibody;C-HA-RAS1 antibody;CTLO antibody;GTPase HRas antibody;GTPase KRas antibody;GTPase NRas antibody;H-Ras-1 antibody;H-RASIDX antibody;Ha-Ras antibody;HAMSV antibody;HRAS antibody;HRAS1 antibody;K RAS2A antibody;K RAS2B antibody;K RAS4A antibody;K RAS4B antibody;K-RAS antibody;KRAS antibody;KRAS1 antibody;KRAS2 antibody;N-RAS antibody;N-terminally processed antibody;NRAS antibody;NRAS1 antibody;p21ras antibody;RASH_HUMAN antibody;RASH1 antibody;RASK2 antibody;Transforming protein p21 antibody;v Ha ras Harvey rat sarcoma viral oncogene homolog antibody;v Ki ras2 Kirsten rat sarcoma viral oncogene homolog antibody;v ras neuroblastoma RAS viral oncogene homolog antibody
SEQUENCE SIMILARITIES
Belongs to the small GTPase superfamily. Ras family.
POST-TRANSLATIONAL MODIFICATION
Palmitoylated by the ZDHHC9-GOLGA7 complex. Depalmitoylated by ABHD17A, ABHD17B and ABHD17C. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.; Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).; Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes.; Phosphorylation at Ser-89 by STK19 enhances NRAS-association with its downstream effectors.
SUBCELLULAR LOCATION
Cytoplasm, Cell membrane, Golgi apparatus.
FUNCTION
Ras, from "Rat sarcoma virus", is a family of related proteins that are expressed in all animal cell lineages and organs. All Ras protein family members belong to a class of protein called small GTPase, and are involved in transmitting signals within cells (cellular signal transduction). Ras is the prototypical member of the Ras superfamily of proteins, which are all related in three-dimensional structure and regulate diverse cell behaviours. When Ras is 'switched on' by incoming signals, it subsequently switches on other proteins, which ultimately turn on genes involved in cell growth, differentiation, and survival. Mutations in Ras genes can lead to the production of permanently activated Ras proteins, which can cause unintended and overactive signaling inside the cell, even in the absence of incoming signals. Because these signals result in cell growth and division, overactive Ras signaling can ultimately lead to cancer. The three Ras genes in humans (HRAS, KRAS, and NRAS) are the most common oncogenes in human cancer.
