Collagen VI Recombinant Rabbit Monoclonal Antibody [SD83-03] (ET1612-91)
Catalog# ET1612-91
Rabbit Monoclonal to Collagen VI
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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Human
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Mouse
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Rat
Lane 1: NIH/3T3 cell lysate (15 µg/Lane)
Lane 2: Human kidney tissue lysate (20 µg/Lane)
Lane 3: Rat heart tissue lysate (20 µg/Lane)
Lane 4: Rat kidney tissue lysate (20 µg/Lane)
Predicted band size: 109 kDa
Observed band size: 145 kDa
Exposure time: 1 minute 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-91) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Collagen VI Recombinant Rabbit Monoclonal Antibody [SD83-03] (ET1612-91)
Immunogen
Recombinant protein within human collagen vi alpha1 aa 17-255 / 1,028.
Host
Rabbit
Positive Control
NIH/3T3 cell lysate, human kidney tissue lysate, rat heart tissue lysate, rat kidney tissue lysate, Hela, A549, HepG2, RH-35, human lung tissue, human liver tissue, mouse colon tissue, human colon tissue, human skin tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SD83-03
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IF-Cell
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1:100-1:500
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IF-Tissue
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1:100-1:500
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IHC-P
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 109 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Collagen VI
SYNONYMS
Alpha 1 (VI) chain (61 AA) antibody;CO6A1_HUMAN antibody;COL6A1 antibody;Collagen alpha-1(VI) chain antibody;Collagen type VI alpha 1 antibody;Collagen VI alpha 1 polypeptide antibody;CollagenVI antibody;Human mRNA for collagen VI alpha 2 C terminal globular domain antibody;OPLL antibody;PP3610 antibody
SEQUENCE SIMILARITIES
Belongs to the type VI collagen family.
POST-TRANSLATIONAL MODIFICATION
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
SUBCELLULAR LOCATION
Extracellular matrix.
FUNCTION
Collagen VI (ColVI) is a type of collagen primarily associated with the extracellular matrix of skeletal muscle. ColVI maintains regularity in muscle function and stabilizes the cell membrane. It is synthesized by a complex, multistep pathway that leads to the formation of a unique network of linked microfilaments located in the extracellular matrix (ECM). ColVI plays a vital role in numerous cell types, including chondrocytes, neurons, myocytes, fibroblasts, and cardiomyocytes. ColVI molecules are made up of three alpha chains: α1(VI), α2(VI), and α3(VI). It is encoded by 6 genes: COL6A1, COL6A2, COL6A3, COL6A4, COL6A5, and COL6A6. The chain lengths of α1(VI) and α2(VI) are about 1,000 amino acids. The chain length of α3(VI) is roughly a third larger than those of α1(VI) and α2(VI), and it consists of several spliced variants within the range of 2,500 to 3,100 amino acids. The first two alpha chains subunits of ColVI have a molecular weight of 140-150 KDa and the third polypeptide chain is larger with a molecular weight of 250-300kDa. ColVI is also found in the skin, lungs, blood vessels, cornea and intervertebral disc. It also forms part of the peripheral nerves, brain, myocardium and adipose tissue.
CITATIONS
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Zhou, S., Ma, Y., Yao, J., Zhao, A., Xie, C., Mi, Y., & Zhang, C.
TGF-β1-induced collagen promotes chicken ovarian follicle development via an intercellular cooperative pattern
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WB
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Hen
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Citation
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Caiqiao Zhang
Transcriptome profiling analysis of underlying regulation of growing follicle development in the chicken
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IHC
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WB
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chicken
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Citation
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