CD10 Recombinant Rabbit Monoclonal Antibody [SN75-07] (ET1611-82)
Catalog# ET1611-82
Rabbit Monoclonal to CD10
Tested for
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WB
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FC
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IHC-P
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IF-Cell
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IF-Tissue
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of CD10 on different lysates with Rabbit anti-CD10 antibody (ET1611-82) at 1/2,000 dilution.
Lane 1: Ramos cell lysate (15 µg/Lane)
Lane 2: Daudi cell lysate (15 µg/Lane)
Lane 3: U-2 OS cell lysate (negative) (15 µg/Lane)
Lane 4: Mouse kidney tissue lysate (20 µg/Lane)
Lane 5: Mouse small intestine tissue lysate (20 µg/Lane)
Lane 6: Rat lung tissue lysate (20 µg/Lane)
Predicted band size: 86 kDa
Observed band size: 100 kDa
Exposure time: 24 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-82) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Ramos cell lysate (15 µg/Lane)
Lane 2: Daudi cell lysate (15 µg/Lane)
Lane 3: U-2 OS cell lysate (negative) (15 µg/Lane)
Lane 4: Mouse kidney tissue lysate (20 µg/Lane)
Lane 5: Mouse small intestine tissue lysate (20 µg/Lane)
Lane 6: Rat lung tissue lysate (20 µg/Lane)
Predicted band size: 86 kDa
Observed band size: 100 kDa
Exposure time: 24 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-82) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.