Laminin beta 1 Recombinant Rabbit Monoclonal Antibody [JM099-03] (ET1703-14)
Catalog# ET1703-14
Rabbit Monoclonal to Laminin beta 1
Tested for
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WB
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IHC-P
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FC
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mIHC
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Laminin beta 1 on different lysates with Rabbit anti-Laminin beta 1 antibody (ET1703-14) at 1/2,000 dilution.
Lane 1: HepG2 cell lysate (15 µg/Lane)
Lane 2: C6 cell lysate (15 µg/Lane)
Lane 3: Mouse stomach tissue lysate (20 µg/Lane)
Lane 4: Rat heart tissue lysate (20 µg/Lane)
Predicted band size: 198 kDa
Observed band size: 250 kDa
Exposure time: 3 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-14) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate (15 µg/Lane)
Lane 2: C6 cell lysate (15 µg/Lane)
Lane 3: Mouse stomach tissue lysate (20 µg/Lane)
Lane 4: Rat heart tissue lysate (20 µg/Lane)
Predicted band size: 198 kDa
Observed band size: 250 kDa
Exposure time: 3 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-14) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.