TIMP3 Recombinant Rabbit Monoclonal Antibody [PSH04-41] (HA722126)
Catalog# HA722126
Rabbit Monoclonal to TIMP3
Tested for
-
WB
-
IF-Cell
Reacts with
-
Human
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of TIMP3 on different lysates with Rabbit anti-TIMP3 antibody (HA722126) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: A431 cell lysate
Lane 3: Human liver tissue lysate (negative)
Lane 4: Rat placenta tissue lysate
Lane 5: Rat spleen tissue lysate
Lane 6: Rat liver tissue lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 24 kDa
Observed band size: 20 kDa
Exposure time: 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722126) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate
Lane 2: A431 cell lysate
Lane 3: Human liver tissue lysate (negative)
Lane 4: Rat placenta tissue lysate
Lane 5: Rat spleen tissue lysate
Lane 6: Rat liver tissue lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 24 kDa
Observed band size: 20 kDa
Exposure time: 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722126) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.