ST2 Mouse Monoclonal Antibody [PSH01-69] (HA601171)
Catalog# HA601171
Mouse Monoclonal to ST2
Tested for
-
WB
-
IF-Cell
-
FC
Reacts with
-
Human
Western blot analysis of ST2 on different lysates with Mouse anti-ST2 antibody (HA601171) at 1/1,000 dilution.
Lane 1: HeLa transfected with FLAG-tagged empty control cell lysate
Lane 2: HeLa transfected with FLAG-tagged ST2 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 63 kDa
Observed band size: 80 kDa
Exposure time: 5 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601171) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa transfected with FLAG-tagged empty control cell lysate
Lane 2: HeLa transfected with FLAG-tagged ST2 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 63 kDa
Observed band size: 80 kDa
Exposure time: 5 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601171) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.