CD31 Recombinant Rabbit Monoclonal Antibody [SU03-59] (ET1608-48)
Catalog# ET1608-48
Rabbit Monoclonal to CD31
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WB
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IHC-P
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FC
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IP
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IF-Cell
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IF-Tissue
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Human
Lysates/proteins at 15 µg/Lane.
Predicted band size: 83 kDa
Observed band size: 130 kDa
Exposure time: 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-48) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CD31 Recombinant Rabbit Monoclonal Antibody [SU03-59] (ET1608-48)
Immunogen
Synthetic peptide within human cd31 aa 689-738 / 738.
Host
Rabbit
Positive Control
THP-1 cell lysate, human tonsil tissue, THP-1.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SU03-59
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IHC-P:1:50-1:1,000
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FC
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1:50-1:100
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IP
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1-2μg/sample
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IF-Cell
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1:100
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IF-Tissue
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1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 83 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CD31
SYNONYMS
Adhesion molecule antibody;CD31 antibody;CD31 antigen antibody;CD31 EndoCAM antibody;EndoCAM antibody;FLJ34100 antibody;FLJ58394 antibody;GPIIA antibody;GPIIA' antibody;PECA1 antibody;PECA1_HUMAN antibody;Pecam 1 antibody;PECAM 1 CD31 EndoCAM antibody;PECAM antibody;PECAM-1 antibody;Pecam1 antibody;Platelet and endothelial cell adhesion molecule 1 antibody;Platelet endothelial cell adhesion molecule antibody;Platelet/endothelial cell adhesion molecule 1 antibody
TISSUE SPECIFICITY
Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Expressed in human umbilical vein endothelial cells (HUVECs) (at protein level). Expressed on neutrophils (at protein level). Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U-937 histiocytic lymphoma cell lines (at protein level).
POST-TRANSLATIONAL MODIFICATION
Phosphorylated on Ser and Tyr residues after cellular activation by src kinases. Upon activation, phosphorylated on Ser-729 which probably initiates the dissociation of the membrane-interaction segment (residues 709-729) from the cell membrane allowing the sequential phosphorylation of Tyr-713 and Tyr-690. Constitutively phosphorylated on Ser-734 in resting platelets. Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation (By similarity). In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.; Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts.
SUBCELLULAR LOCATION
Cell junction. Cell membrane. Membrane.
FUNCTION
PECAM-1 is a cell-cell adhesion protein which interacts with other PECAM-1 molecules through homophilic interactions or with non-PECAM-1 molecules through heterophilic interactions. Homophilic interactions between PECAM-1 molecules are mediated by antiparallel interactions between extracellular Ig-like domain 1 and Ig-like domain 2. These interactions are regulated by the level of PECAM-1 expression. Homophilic interactions occur, only when the surface expression of PECAM-1 is high. Otherwise, when expression is low, heterophilic interactions occur.
CITATIONS
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Che, L. H., Liu, J. W., Huo, J. P., Luo, R., Xu, R. M., He, C., Li, Y. Q., Zhou, A. J., Huang, P., Chen, Y. Y., Ni, W., Zhou, Y. X., Liu, Y. Y., Li, H. Y., Zhou, R., Mo, H., & Li, J. M.
A single-cell atlas of liver metastases of colorectal cancer reveals reprogramming of the tumor microenvironment in response to preoperative chemotherapy
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IHC
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Human
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Citation
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Min Han,JianQing Gao
Targeting Tumor Hypoxia with Stimulus-Responsive Nanocarriers in Overcoming Drug Resistance and Monitoring Anticancer Efficacy
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WB
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Mouse
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Citation
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Microglia Regulate Blood–Brain Barrier Integrity via MiR‐126a‐5p/MMP9 Axis during Inflammatory Demyelination
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IF
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Citation
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Huang Xiuhong,et al
Polyphenol-Based Photothermal Nanoparticles with Sprayable Capability for Self-regulation of Microenvironment to Accelerate Diabetic Wound Healing
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IF
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Human
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Citation
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Chen, Y., Lu, Z., Qi, C., Yu, C., Li, Y., Huan, W., Wang, R., Luo, W., Shen, D., Ding, L., Ren, L., Xie, H., Xue, D., Wang, M., Ni, K., Xia, L., Qian, J., & Li, G.
N6-methyladenosine-modified TRAF1 promotes sunitinib resistance by regulating apoptosis and angiogenesis in a METTL14-dependent manner in renal cell carcinoma
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IHC
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Human
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Citation
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Yes-associated protein promotes endothelial-to-mesenchymal transition of endothelial cells in choroidal neovascularization fibrosis
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WB
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Human
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Citation
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Huang Xiuhong,Fu Meimei,Lu Min,et al
Polyphenol-Based Photothermal Nanoparticles with Sprayable Capability for Self-regulation of Microenvironment to Accelerate Diabetic Wound Healing
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IF
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Human
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Citation
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