SIRT7 Recombinant Rabbit Monoclonal Antibody [PSH09-17] (HA723075)
Catalog# HA723075
Rabbit Monoclonal to SIRT7
Tested for
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WB
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IF-Cell
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IHC-P
-
IP
-
ChIP
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of SIRT7 on different lysates with Rabbit anti-SIRT7 antibody (HA723075) at 1/2,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: HeLa cell lysate
Lane 3: U-2 OS cell lysate
Lane 4: Neuro-2a cell lysate
Lane 5: C6 cell lysate
Lane 6: Mouse liver tissue lysate
Lane 7: Rat spleen tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723075) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HEK-293 cell lysate
Lane 2: HeLa cell lysate
Lane 3: U-2 OS cell lysate
Lane 4: Neuro-2a cell lysate
Lane 5: C6 cell lysate
Lane 6: Mouse liver tissue lysate
Lane 7: Rat spleen tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723075) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.