Integrin beta 3 Recombinant Rabbit Monoclonal Antibody [PSH09-27] (HA723085)
Catalog# HA723085
Rabbit Monoclonal to Integrin beta 3
Tested for
-
WB
-
IHC-P
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Integrin beta 3 on different lysates with Rabbit anti-Integrin beta 3 antibody (HA723085) at 1/5,000 dilution.
Lane 1: U-87 MG cell lysate (20 µg/Lane)
Lane 2: LNCaP cell lysate (low expression) (20 µg/Lane)
Lane 3: HUVEC cell lysate (20 µg/Lane)
Lane 4: C2C12 cell lysate (20 µg/Lane)
Lane 5: Mouse spleen tissue lysate (40 µg/Lane)
Lane 6: Rat spleen tissue lysate (40 µg/Lane)
Predicted band size: 87 kDa
Observed band size: 100 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723085) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: U-87 MG cell lysate (20 µg/Lane)
Lane 2: LNCaP cell lysate (low expression) (20 µg/Lane)
Lane 3: HUVEC cell lysate (20 µg/Lane)
Lane 4: C2C12 cell lysate (20 µg/Lane)
Lane 5: Mouse spleen tissue lysate (40 µg/Lane)
Lane 6: Rat spleen tissue lysate (40 µg/Lane)
Predicted band size: 87 kDa
Observed band size: 100 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723085) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.