Hsp27 Mouse Monoclonal Antibody [10-8] (M1505-3)

UNIPROT #

P04792

PROTEIN NAME

Hsp27

SYNONYMS

Heat shock 27kDa protein antibody;28 kDa heat shock protein antibody;CMT2F antibody;DKFZp586P1322 antibody;epididymis secretory protein Li 102 antibody;Estrogen regulated 24 kDa protein antibody;Estrogen-regulated 24 kDa protein antibody;Heat shock 25kDa protein 1 antibody;Heat shock 27 kDa protein antibody;Heat shock 27kD protein 1 antibody;Heat shock 27kDa protein 1 antibody;Heat shock 28kDa protein 1 antibody;Heat Shock Protein 27 antibody;Heat shock protein beta 1 antibody;Heat shock protein beta-1 antibody;heat shock protein family B (small) member 1 antibody;HEL-S-102 antibody;HMN2B antibody;HS.76067 antibody;Hsp 25 antibody;HSP 27 antibody;Hsp 28 antibody;Hsp B1 antibody;Hsp25 antibody;HSP27 antibody;Hsp28 antibody;HspB1 antibody;HSPB1_HUMAN antibody;SRP27 antibody;Stress responsive protein 27 antibody;Stress-responsive protein 28 antibody

SEQUENCE SIMILARITIES

Belongs to the small heat shock protein (HSP20) family.

TISSUE SPECIFICITY

Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated upon exposure to protein kinase C activators and heat shock. Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement.

SUBCELLULAR LOCATION

Cytoplasm, nucleus

FUNCTION

Hsp27 is a chaperone of the sHsp(small heat shock protein) group among ubiquitin, α-crystallin,Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and?cell differentiation. They also take part in signal transduction. The main function of Hsp27 is to provide thermotolerance in vivo, cytoprotection, and support of cell survival under stress conditions. More specialized functions of Hsp27 are manifold and complex. In vitro it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding by the Hsp70-complex. Hsp27 is also involved in the apoptotic signalling pathway. Hsp27 interacts with the outer mitochondrial membranes and interferes with the activation of?cytochrome c/Apaf-1/dATP complex and therefore inhibits the activation of procaspase-9.