MMP-1 Recombinant Rabbit Monoclonal Antibody [PSH09-56] (HA723118)
Catalog# HA723118
Rabbit Monoclonal to MMP-1
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of MMP-1 on different lysates with Rabbit anti-MMP-1 antibody (HA723118) at 1/2,000 dilution.
Lane 1: MDA-MB-231 cell lysate
Lane 2: U-87 MG cell lysate
Lane 3: HT-29 cell lysate (negative)
Lane 4: HUVEC cell lysate
Lane 5: A375 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 27 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723118) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: MDA-MB-231 cell lysate
Lane 2: U-87 MG cell lysate
Lane 3: HT-29 cell lysate (negative)
Lane 4: HUVEC cell lysate
Lane 5: A375 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 27 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723118) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.