Alpha 1 Acid Glycoprotein Mouse Monoclonal Antibody [A4-G2-A3] (EM1510-42)
Catalog# EM1510-42
Mouse Monoclonal to Alpha 1 Acid Glycoprotein
Tested for
-
WB
-
IHC-P
-
FC
Reacts with
-
Human
-
Rat
-
Mouse
Western blot analysis of ORM1 on different lysates with Mouse anti-ORM1 antibody (EM1510-42) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: THP-1 cell lysate (20 µg/Lane)
Lane 4: HCT 116 cell lysate (20 µg/Lane)
Lane 5: 293T cell lysate (20 µg/Lane)
Lane 6: NIH:OVCAR-3 cell lysate (20 µg/Lane)
Lane 7: Human liver tissue lysate (40 µg/Lane)
Predicted band size: 24 kDa
Observed band size: 37/45 kDa
Exposure time: 1 minute 59 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1510-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: THP-1 cell lysate (20 µg/Lane)
Lane 4: HCT 116 cell lysate (20 µg/Lane)
Lane 5: 293T cell lysate (20 µg/Lane)
Lane 6: NIH:OVCAR-3 cell lysate (20 µg/Lane)
Lane 7: Human liver tissue lysate (40 µg/Lane)
Predicted band size: 24 kDa
Observed band size: 37/45 kDa
Exposure time: 1 minute 59 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1510-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.