BNP Recombinant Rabbit Monoclonal Antibody [JE30-94] (HA721946)
Catalog# HA721946
Rabbit Monoclonal to BNP
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of BNP on different lysates with Rabbit anti-BNP antibody (HA721946) at 1/1,000 dilution.
Lane 1: SW480 cell lysate (20 µg/Lane)
Lane 2: U-87 MG cell lysate (20 µg/Lane)
Lane 3: U-2 OS cell lysate (20 µg/Lane)
Lane 4: Mouse brain tissue lysate (30 µg/Lane)
Lane 5: Rat brain tissue lysate (30 µg/Lane)
Predicted band size: 15 kDa
Observed band size: 37 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721946) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: SW480 cell lysate (20 µg/Lane)
Lane 2: U-87 MG cell lysate (20 µg/Lane)
Lane 3: U-2 OS cell lysate (20 µg/Lane)
Lane 4: Mouse brain tissue lysate (30 µg/Lane)
Lane 5: Rat brain tissue lysate (30 µg/Lane)
Predicted band size: 15 kDa
Observed band size: 37 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721946) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.