PAI1 Mouse Monoclonal Antibody [A9G8] (HA601152)
Catalog# HA601152
Mouse Monoclonal to PAI1
Tested for
-
WB
-
IHC-P
-
IF-Tissue
-
FC
Reacts with
-
Human
-
Mouse
-
Rat
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of PAI1 on different lysates with Mouse anti-PAI1 antibody (HA601152) at 1/1,000 dilution.
Lane 1: no heat NIH/3T3 cell lysate (20 µg/Lane)
Lane 2: no heat NIH/3T3 serum starved for 4 hours then treated with 10ng/mL TGF-β for 21 hours add 300ng/mL BFA for 18 hours cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 serum starved for 4 hours then treated with 10ng/mL TGF-β for 21 hours add 300ng/mL BFA for 18 hours cell lysate (20 µg/Lane)
Lane 5: HUVEC cell lysate (20 µg/Lane)
Lane 6: Mouse placenta tissue lysate (40 µg/Lane)
Lane 7: Rat placenta tissue lysate (40 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 45 kDa
Observed band size: 45/100 kDa
Exposure time: 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601152) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (NBI02H) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: no heat NIH/3T3 cell lysate (20 µg/Lane)
Lane 2: no heat NIH/3T3 serum starved for 4 hours then treated with 10ng/mL TGF-β for 21 hours add 300ng/mL BFA for 18 hours cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 serum starved for 4 hours then treated with 10ng/mL TGF-β for 21 hours add 300ng/mL BFA for 18 hours cell lysate (20 µg/Lane)
Lane 5: HUVEC cell lysate (20 µg/Lane)
Lane 6: Mouse placenta tissue lysate (40 µg/Lane)
Lane 7: Rat placenta tissue lysate (40 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 45 kDa
Observed band size: 45/100 kDa
Exposure time: 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601152) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (NBI02H) at 1:100,000 dilution was used for 1 hour at room temperature.
