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ULK1 Recombinant Rabbit Monoclonal Antibody [JA58-36] (ET1704-63)

Price: Regular price $385.00

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Catalog# ET1704-63

Rabbit Monoclonal to ULK1

Tested for

  • WB

  • IHC-P

Reacts with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE

Safety datasheet

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Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

ULK1 Recombinant Rabbit Monoclonal Antibody [JA58-36] (ET1704-63)

Immunogen

Synthetic peptide within human ulk1 aa 930-979 / 1050.

Host

Rabbit

Positive Control

A-172 cell lysate, SH-SY5Y cell lysate, U-2 OS cell lysate, MCF7 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, human spleen tissue, rat skeletal muscle tissue, human colon carcinoma tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA58-36

RRID

AB_3070509

APPLICATION DILUTION

  • WB

  • 1:5,000

  • IHC-P

  • 1:50-1:200

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 113 kDa

Isotype

IgG

TARGET

UNIPROT #

O75385

PROTEIN NAME

ULK1

SYNONYMS

ATG 1 antibody;ATG1 antibody;ATG1 autophagy related 1 homolog antibody;ATG1A antibody;Autophagy related protein 1 homolog antibody;Autophagy-related protein 1 homolog antibody;FLJ38455 antibody;FLJ46475 antibody;hATG1 antibody;KIAA0722 antibody;Serine/threonine protein kinase ULK1 antibody;Serine/threonine protein kinase Unc51.1 antibody;Serine/threonine-protein kinase ULK1 antibody;ULK 1 antibody;ULK1 antibody;ULK1_HUMAN antibody;Unc 51 (C. elegans) like kinase 1 antibody;UNC 51 antibody;Unc 51 like kinase 1 antibody;Unc-51 like kinase 1 (C. elegans) antibody;Unc-51-like kinase 1 antibody;UNC51 antibody;UNC51, C. elegans, homolog of antibody;Unc51.1 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.

TISSUE SPECIFICITY

Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.

POST-TRANSLATIONAL MODIFICATION

Autophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity). In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.; Acetylated by KAT5/TIP60 under autophagy induction, promoting protein kinase activity.

SUBCELLULAR LOCATION

Cytosol, Preautophagosomal structure.

FUNCTION

Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy.

CITATIONS

  • Wu, C. J., Fu, F. D., Qin, F., Ma, M., Li, T., Wei, S. Z., Yu, B. T., Yang, X. Z., & Yuan, J. H.

    Vacuum therapy ameliorates erectile dysfunction in bilateral cavernous nerve crush rats by inhibiting apoptosis and activating autophagy

  • Hu, Yeting;Qian, Yucheng;Wei, Jingsun;Jin, Tian;Kong, Xiangxing;Cao, Hongfeng;Ding, Kefeng

    The Disulfiram/Copper Complex Induces Autophagic Cell Death in Colorectal Cancer by Targeting ULK1

  • Zhang, X., Yang, Z., Pan, T., Long, X., Sun, Q., Wang, P. H., Li, X., & Kuang, E.

    SARS-CoV-2 ORF3a induces RETREG1/FAM134B dependent reticulophagy and triggers sequential ER stress and inflammatory responses during SARS-CoV-2 infection

  • Inhibition of STX17–SNAP29–VAMP8 complex formation by costunolide sensitizes ovarian cancer cells to cisplatin via the AMPK/mTOR signaling pathway

  • miR-145-5p affects autophagy by targeting CaMKIIδ in atherosclerosis

  • Xiang, X., Tian, Y., Hu, J., Xiong, R., Bautista, M., Deng, L., Yue, Q., Li, Y., Kuang, W., Li, J., Liu, K., Yu, C., & Feng, G.

    Fangchinoline exerts anticancer effects on colorectal cancer by inducing autophagy via regulation AMPK/mTOR/ULK1 pathway. Biochemical pharmacology, 186, 114475.

  • Wu, Y., Hu, A., Shu, X., Huang, W., Zhang, R., Xu, Y., & Yang, C.

    Lactobacillus plantarum postbiotics trigger AMPK-dependent autophagy to suppress Salmonella intracellular infection and NLRP3 inflammasome activation

  • Feng Yuemei,et al

    Cafestol inhibits colon cancer cell proliferation and tumor growth in xenograft mice by activating LKB1/AMPK/ULK1-dependent autophagy

  • Aixin Hu , Wenxia Huang , Xin Shu , Shiyue Ma , Caimei Yang , Ruiqiang Zhang , Xiao Xiao , Yanping Wu

    Lactiplantibacillus plantarum Postbiotics Suppress Salmonella Infection via Modulating Bacterial Pathogenicity, Autophagy and Inflammasome in Mice

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