Smad4 Recombinant Rabbit Monoclonal Antibody [SP05-05] (ET1604-12)
Catalog# ET1604-12
Rabbit Monoclonal to Smad4
Tested for
-
WB
-
IF-Cell
-
IF-Tissue
-
IHC-P
-
FC
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of Smad4 on different lysates with Rabbit anti-Smad4 antibody (ET1604-12) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HCT 116 cell lysate
Lane 3: C6 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 60 kDa
Observed band size: 70 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1604-12) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: HCT 116 cell lysate
Lane 3: C6 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 60 kDa
Observed band size: 70 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1604-12) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
