Pan-Actin Mouse Monoclonal Antibody [A2E1] (HA600032)
Catalog# HA600032
Mouse Monoclonal to Pan-Actin
Tested for
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WB
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IHC-P
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IF-Cell
Reacts with
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Human
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Mouse
-
Rat
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of Pan-Actin on different lysates with Mouse anti-Pan-Actin antibody (HA600032) at 1/1,000 dilution.
Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: MCF7 cell lysate (20 µg/Lane)
Lane 3: HEK-293 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (20 µg/Lane)
Lane 5: PC-12 cell lysate (20 µg/Lane)
Lane 6: Mouse smooth muscle tissue lysate (40 µg/Lane)
Lane 7: Rat heart tissue lysate (40 µg/Lane)
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 9 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600032) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: MCF7 cell lysate (20 µg/Lane)
Lane 3: HEK-293 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (20 µg/Lane)
Lane 5: PC-12 cell lysate (20 µg/Lane)
Lane 6: Mouse smooth muscle tissue lysate (40 µg/Lane)
Lane 7: Rat heart tissue lysate (40 µg/Lane)
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 9 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600032) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
