HIF-1 alpha Recombinant Rabbit Monoclonal Antibody [JE75-33] (HA721997)
Catalog# HA721997
Rabbit Monoclonal to HIF-1 alpha
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WB
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IF-Cell
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Human
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Mouse
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Rat
Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (HA721997) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 0.5mM CoCl2 for 6 hours cell lysate
Lane 3: HepG2 cell lysate
Lane 4: HepG2 treated with 100μM CoCl2 for 4 hours cell lysate
Lane 5: C2C12 cell lysate
Lane 6: C2C12 treated with 100μM CoCl2 for 4 hours cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 93 kDa
Observed band size: 120 kDa
Exposure time: 40 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721997) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
HIF-1 alpha Recombinant Rabbit Monoclonal Antibody [JE75-33] (HA721997)
Immunogen
Recombinant protein within human hif-1 alpha aa 251-550 / 826.
Host
Rabbit
Positive Control
HeLa treated with 0.5mM CoCl2 for 6 hours cell lysate, HepG2 treated with 100μM CoCl2 for 4 hours cell lysate, C2C12 treated with 100μM CoCl2 for 4 hours cell lysate, HeLa cells treated with or without 500μM CoCl2 for 24 hours, human kidney tissue, mouse kidney tissue, rat kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE75-33
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IF-Cell
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 93 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
HIF-1 alpha
SYNONYMS
ARNT interacting protein antibody;ARNT-interacting protein antibody;Basic helix loop helix PAS protein MOP1 antibody;Basic-helix-loop-helix-PAS protein MOP1 antibody;bHLHe78 antibody;Class E basic helix-loop-helix protein 78 antibody;HIF 1A antibody;HIF 1alpha antibody;HIF-1-alpha antibody;HIF-1alpha antibody;HIF-alpha antibody;HIF1 A antibody;HIF1 Alpha antibody;HIF1 antibody;HIF1-alpha antibody;HIF1A antibody;HIF1A_HUMAN antibody;hifla antibody;Hypoxia inducible factor 1 alpha antibody;Hypoxia inducible factor 1 alpha isoform I.3 antibody;Hypoxia inducible factor 1 alpha subunit antibody;Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor antibody;Hypoxia inducible factor 1, alpha subunit (basic helix loop helix transcription factor) antibody;Hypoxia inducible factor1alpha antibody;Hypoxia-inducible factor 1-alpha antibody;Member of PAS protein 1 antibody;Member of PAS superfamily 1 antibody;Member of the PAS Superfamily 1 antibody;MOP 1 antibody;MOP1 antibody;PAS domain-containing protein 8 antibody;PASD 8 antibody;PASD8 antibody
SUBCELLULAR LOCATION
Cytoplasm, Nucleus, Nucleus speckle.
FUNCTION
Hypoxia-inducible factor 1-alpha, also known as HIF-1-alpha, is a subunit of a heterodimeric transcription factor hypoxia-inducible factor 1 (HIF-1) that is encoded by the HIF1A gene. The Nobel Prize in Physiology or Medicine 2019 was awarded for the discovery of HIF. HIF1A is a basic helix-loop-helix PAS domain containing protein, and is considered as the master transcriptional regulator of cellular and developmental response to hypoxia. The dysregulation and overexpression of HIF1A by either hypoxia or genetic alternations have been heavily implicated in cancer biology, as well as a number of other pathophysiologies, specifically in areas of vascularization and angiogenesis, energy metabolism, cell survival, and tumor invasion. Two other alternative transcripts encoding different isoforms have been identified.
CITATIONS
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