HIF-1 beta Recombinant Rabbit Monoclonal Antibody [ST05-25] (ET1609-67)
Catalog# ET1609-67
Rabbit Monoclonal to HIF-1 beta
Tested for
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WB
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IHC-P
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
Western blot analysis of HIF-1 beta on different lysates with Rabbit anti-HIF-1 beta antibody (ET1609-67) at 1/5,000 dilution.
Lane 1: A549-si NT cell lysate
Lane 2: A549-si HIF-1 beta cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 87 kDa
Observed band size: 72/87 kDa
Exposure time: 1 minute 20 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-67) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A549-si NT cell lysate
Lane 2: A549-si HIF-1 beta cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 87 kDa
Observed band size: 72/87 kDa
Exposure time: 1 minute 20 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-67) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.