PHD3 Recombinant Rabbit Monoclonal Antibody [JE60-82] (HA721929)
Catalog# HA721929
Rabbit Monoclonal to PHD3
Tested for
-
WB
Reacts with
-
Human
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of PHD3 on different lysates with Rabbit anti-PHD3 antibody (HA721929) at 1/1,000 dilution.
Lane 1: Mouse liver tissue lysate (40 µg/Lane)
Lane 2: K-562 cell lysate (20 µg/Lane)
Lane 3: A375 cell lysate (20 µg/Lane)
Lane 4: HEK-293 cell lysate (20 µg/Lane)
Predicted band size: 27 kDa
Observed band size: 30 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721929) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse liver tissue lysate (40 µg/Lane)
Lane 2: K-562 cell lysate (20 µg/Lane)
Lane 3: A375 cell lysate (20 µg/Lane)
Lane 4: HEK-293 cell lysate (20 µg/Lane)
Predicted band size: 27 kDa
Observed band size: 30 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721929) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.