LXR alpha Recombinant Rabbit Monoclonal Antibody [JA20-38] (ET1704-51)
Catalog# ET1704-51
Rabbit Monoclonal to LXR alpha
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
Lane 1: Jurkat cell lysate (15 µg/Lane)
Lane 2: HeLa cell lysate (15 µg/Lane)
Lane 3: Mouse stomach tissue lysate (20 µg/Lane)
Lane 4: Mouse lung tissue lysate (20 µg/Lane)
Lane 5: Rat liver tissue lysate (20 µg/Lane)
Lane 6: Rat lung tissue lysate (20 µg/Lane)
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-51) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
LXR alpha Recombinant Rabbit Monoclonal Antibody [JA20-38] (ET1704-51)
Immunogen
Synthetic peptide within human lxr alpha aa 49-98 / 447.
Host
Rabbit
Positive Control
Jurkat cell lysate, HeLa cell lysate, Mouse stomach tissue lysate, Mouse lung tissue lysate, Rat liver tissue lysate, Rat lung tissue lysate, HepG2 cell lysate, Huh7 cell lysate, HepG2, human liver tissue, mouse liver tissue, rat liver tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JA20-38
RRID
APPLICATION DILUTION
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WB
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1:5,000-1:10,000
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IF-Cell
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1:50-1:100
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IHC-P
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1:200-1:1,000
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 50 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
LXR alpha
SYNONYMS
Liver X receptor alpha antibody;LXR a antibody;LXRA antibody;NR1H3 antibody;NR1H3_HUMAN antibody;Nuclear receptor subfamily 1 group H member 3 antibody;Oxysterols receptor LXR alpha antibody;Oxysterols receptor LXR-alpha antibody;RLD 1 antibody;RLD1 antibody
SEQUENCE SIMILARITIES
Belongs to the nuclear hormone receptor family. NR1 subfamily.
TISSUE SPECIFICITY
Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.
POST-TRANSLATIONAL MODIFICATION
Ubiquitinated leading to its degradation by the proteasome.
SUBCELLULAR LOCATION
Nucleus, Cytoplasm.
FUNCTION
Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.
CITATIONS
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Gao Yinhuang,et al
Buqi Huoxue Tongnao prescription protects against chronic cerebral hypoperfusion via regulating PI3K/AKT and LXRα/CYP7A1 signaling pathways
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WB
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Rat
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Citation
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Wang, M. S., Han, Q. S., Jia, Z. R., Chen, C. S., Qiao, C., Liu, Q. Q., Zhang, Y. M., Wang, K. W., Wang, J., Xiao, K., & Ding, X. S.
PPARα agonist fenofibrate relieves acquired resistance to gefitinib in non-small cell lung cancer by promoting apoptosis via PPARα/AMPK/AKT/FoxO1 pathway. Acta pharmacologica Sinica, 10.1038/s41401-021-00638-z. Advance online publication.
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WB
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Human
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Citation
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Mi-Zhuan Li ,et al
Novel Guanidinium-Functionalized Stigmasterol for Bile Salt Binding and Serum Cholesterol Reduction: Synthesis, Interaction Mechanisms, and In Vivo Function
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WB
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Mouse
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Citation
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