ACSL1 Mouse Monoclonal Antibody [A9B5] (HA601112)
Catalog# HA601112
Mouse Monoclonal to ACSL1
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Knock-Out or Knock-Down validated
Western blot analysis of ACSL1 on different lysates with Mouse anti-ACSL1 antibody (HA601112) at 1/5,000 dilution.
Lane 1: A549-si NT cell lysate
Lane 2: A549-si ACSL1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 78 kDa
Observed band size: 70 kDa
Exposure time: 2 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601112) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A549-si NT cell lysate
Lane 2: A549-si ACSL1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 78 kDa
Observed band size: 70 kDa
Exposure time: 2 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601112) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.