Heme Oxygenase 1 (HO-1) Recombinant Rabbit Monoclonal Antibody [SP08-07] (ET1604-45)
Catalog# ET1604-45
Rabbit Monoclonal to Heme Oxygenase 1(HO-1)
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WB
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IHC-P
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IP
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FC
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IF-Tissue
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Human
Cells were fixed and permeabilized. Then stained with the primary antibody (ET1604-45, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Heme Oxygenase 1 (HO-1) Recombinant Rabbit Monoclonal Antibody [SP08-07] (ET1604-45)
Immunogen
Synthetic peptide within human ho-1 aa 227-276 / 288.
Host
Rabbit
Positive Control
Human spleen tissue, human liver tissue, Jurkat.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SP08-07
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:50-1:1,000
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FC
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1:500-1:1,000
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IP
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Use at an assay dependent concentration.
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IF-Tissue
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 33 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Heme Oxygenase 1(HO-1)
SYNONYMS
32 kD antibody;bK286B10 antibody;D8Wsu38e antibody;heat shock protein 32 kD antibody;heat shock protein 32kD antibody;Heat shock protein antibody;Heme oxygenase (decycling) 1 antibody;Heme oxygenase 1 antibody;Hemox antibody;HMOX 1 antibody;Hmox antibody;Hmox1 antibody;HMOX1_HUMAN antibody;HO 1 antibody;HO antibody;HO-1 antibody;HO1 antibody;Hsp32 antibody
SEQUENCE SIMILARITIES
Belongs to the heme oxygenase family.
TISSUE SPECIFICITY
Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level).
SUBCELLULAR LOCATION
Endoplasmic reticulum membrane.
FUNCTION
Heme oxygenases are microsomal enzymes that cleave heme to produce the antioxidant biliverdin, inorganic iron and carbon monoxide (CO). The activity of Heme Oxygenase 1 (HO-1), also designated HSP 32, is highly inducible in response to numerous stimuli, including heme, heavy metals, hormones and oxidative stress. Heme Oxygenase 2, in contrast, appears to be constituitively expressed in mammalian tissues. Heme Oxygenase 2 is involved in the production of carbon monoxide (CO) in brain, where CO is thought to act as a neurotransmitter. The CO signaling system closely parallels the signaling pathway involving nitric oxide, and regulation of the two systems is closely linked. Heme Oxygenase 3 is found in the spleen, liver, thymus, prostate, heart, kidney, brain and testis. A poor heme catalyst, Heme Oxygenase 3 has two heme regulatory motifs that may be involved in heme binding.
CITATIONS
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Iron-overload-induced ferroptosis in mouse cerebral toxoplasmosis promotes brain injury and could be inhibited by Deferiprone
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WB
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Mouse
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Citation
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Yizhen Wang
Cathelicidin-WA attenuates LPS-induced inflammation and redox imbalance through activation of AMPK signaling
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WB
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Mouse
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Citation
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Zhang Yiyuan,et al
SBFI26 induces triple- negative breast cancer cells ferroptosis via lipid peroxidation
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WB
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Human
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Citation
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Identification, in silico selection, and mechanism study of novel antioxidant peptides derived from the rice bran protein hydrolysates
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WB
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Human
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Citation
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Cen Qingyan,et al
CLPs-miR-103a-2-5p inhibits proliferation and promotes cell apoptosis in AML cells by targeting LILRB3 and Nrf2/HO-1 axis, regulating CD8 + T cell response
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WB
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IHC
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Human
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Citation
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Han, H., Xu, B., Amin, A., Li, H., Yu, X., Gong, M., & Zhang, L.
Quercetin‑3‑O‑α‑L‑rhamnopyranoside derived from the leaves of Lindera aggregata (Sims) Kosterm. evokes the autophagy‑induced nuclear factor erythroid 2‑related factor 2 antioxidant pathway in human umbilical vein endothelial cells. International journal of molecular medicine, 43(1), 461–474.
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WB
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Human
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Citation
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Shikonin and cisplatin synergistically overcome cisplatin resistance of ovarian cancer by inducing ferroptosis via upregulation of HMOX1 to promote Fe2+ accumulation
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WB
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Human
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Citation
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Silencing of HMOX1 enhances apoptosis caused by arsenic trioxide in HCC
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WB
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Human
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Citation
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Jiang SJ, Xiao X, Li J, Mu Y
Lycium barbarum polysaccharide-glycoprotein ameliorates ionizing radiation-induced epithelial injury by regulating oxidative stress and ferroptosis via the Nrf2 pathway
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WB
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Rat
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Human
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Citation
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Yuan, H., Xu, Y., Luo, Y., Zhang, J. R., Zhu, X. X., & Xiao, J. H.
Ganoderic acid D prevents oxidative stress-induced senescence by targeting 14-3-3ε to activate CaM/CaMKII/NRF2 signaling pathway in mesenchymal stem cells
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WB
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Mouse
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Citation
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Protective effect of rutin on ferroptosis‐induced oxidative stress in aging laying hens through Nrf2/HO‐1 signaling
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WB
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Citation
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Modified Montmorillonite Improved Growth Performance of Broilers by Modulating Intestinal Microbiota and Enhancing Intestinal Barriers, Anti-Inflammatory Response, and Antioxidative Capacity
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WB
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Chicken
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Citation
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Shao Chunyan,et al
Selenium nanoparticles alleviates cadmium induced hepatotoxicity by inhibiting ferroptosis and oxidative stress in vivo and in vitro
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WB
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Rat
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Citation
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Liu Y, Lin M, Mu X, et al
Protective effect of solanesol in glucose-induced hepatocyte injury: Mechanistic insights on oxidative stress and mitochondrial preservation
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WB
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Human
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Citation
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Identification and in silico analysis of novel antioxidant peptides in broken rice protein hydrolysate and its cytoprotective effect against H2O2-induced 2BS cell model
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WB
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Human
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Citation
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