LYVE1 Recombinant Rabbit Monoclonal Antibody [JF0979] (ET1702-29)
Catalog# ET1702-29
Rabbit Monoclonal to LYVE1
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WB
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Human
Lane 1: MCF-7 cell lysate
Lane 2: SW480 cell lysate
Lane 3: A431 cell lysate
Lane 4: HUVEC cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-29) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
LYVE1 Recombinant Rabbit Monoclonal Antibody [JF0979] (ET1702-29)
Immunogen
Synthetic peptide within human lyve1 aa 36-72 / 322.
Host
Rabbit
Positive Control
MCF-7 cell lysate, SW480 cell lysate, A431 cell lysate, HUVEC cell lysate, A431, HUVEC, SW480.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JF0979
RRID
APPLICATION DILUTION
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WB
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1:2,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 35 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
LYVE1
SYNONYMS
Cell surface retention sequence-binding protein 1 antibody;CRSBP 1 antibody;CRSBP-1 antibody;CRSBP1 antibody;extracellular link domain containing 1 antibody;extracellular link domain-containing 1 antibody;Extracellular link domain-containing protein 1 antibody;HAR antibody;Hyaluronic acid receptor antibody;Lymphatic endothelium specific hyaluronan receptor antibody;lymphatic vessel endothelial hyaluronan receptor 1 antibody;Lymphatic vessel endothelial hyaluronic acid receptor 1 antibody;LYVE 1 antibody;LYVE-1 antibody;LYVE1 antibody;LYVE1_HUMAN antibody;XLKD1 antibody
TISSUE SPECIFICITY
Mainly expressed in endothelial cells lining lymphatic vessels.
POST-TRANSLATIONAL MODIFICATION
O-glycosylated.
SUBCELLULAR LOCATION
Membrane.
FUNCTION
Lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) is expressed on the cell surface as a protein that is reduced by glycosidase treatment. LYVE-1 is abundant in spleen, lymph node, heart, lung and fetal liver, and is less abundant in appendix, bone marrow, placenta, muscle and adult liver. Expression of LYVE-1 is largely restricted to endothelial cells lining lymphatic vessels and splenic sinusoidal endothelial cells. LYVE-1 binds to both soluble and immobilized hyaluronan (HA) with greater specificity than HCAM. Like HCAM, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike HCAM, the LYVE-1 molecule co-localizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves. LYVE-1 is used as a marker to study tumor lymphangiogenesis, which is an important area of investigation.
CITATIONS
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Suppression of VEGFD expression by S-nitrosylation promotes the development of lung adenocarcinoma
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IF-tissue
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Mouse
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Citation
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