DAP Kinase 1 Recombinant Mouse Monoclonal Antibody [12B2-R] (HA601162)
Catalog# HA601162
Mouse Monoclonal to DAP Kinase 1
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of DAP Kinase 1 on different lysates with Mouse anti-DAP Kinase 1 antibody (HA601162) at 1/2,000 dilution.
Lane 1: A549 cell lysate
Lane 2: TF-1 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Human brain tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 160 kDa
Observed band size: 160 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601162) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: A549 cell lysate
Lane 2: TF-1 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Human brain tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 160 kDa
Observed band size: 160 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601162) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.