Aurora A Recombinant Rabbit Monoclonal Antibody [ST46-07] (ET1609-22)

UNIPROT #

O14965

PROTEIN NAME

Aurora A

SYNONYMS

AIK antibody;ARK-1 antibody;ARK1 antibody;AURA antibody;Aurka antibody;Aurora 2 antibody;Aurora A antibody;Aurora kinase A antibody;Aurora-related kinase 1 antibody;Aurora/IPL1 like kinase antibody;Aurora/IPL1-related kinase 1 antibody;AURORA2 antibody;Breast tumor-amplified kinase antibody;BTAK antibody;hARK1 antibody;IAK antibody;IPL1 related kinase antibody;MGC34538 antibody;OTTHUMP00000031340 antibody;OTTHUMP00000031341 antibody;OTTHUMP00000031342 antibody;OTTHUMP00000031343 antibody;OTTHUMP00000031344 antibody;OTTHUMP00000031345 antibody;OTTHUMP00000166071 antibody;OTTHUMP00000166072 antibody;PPP1R47 antibody;Protein phosphatase 1, regulatory subunit 47 antibody;Serine/threonine kinase 15 antibody;Serine/threonine kinase 6 antibody;Serine/threonine-protein kinase 15 antibody;Serine/threonine-protein kinase 6 antibody;Serine/threonine-protein kinase aurora-A antibody;STK15 antibody;STK6 antibody;STK6_HUMAN antibody;STK7 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.

TISSUE SPECIFICITY

Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.

POST-TRANSLATIONAL MODIFICATION

Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Thr-288 can be phosphorylated by several kinases, including PAK and PKA. Protein phosphatase type 1 (PP1) binds AURKA and inhibits its activity by dephosphorylating Thr-288 during mitosis. Phosphorylation at Ser-342 decreases the kinase activity. PPP2CA controls degradation by dephosphorylating Ser-51 at the end of mitosis.; Ubiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL7) during mitosis, leading to its degradation by the proteasome (By similarity). Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome. Ubiquitinated by the CUL3-KLHL18 ligase leading to its activation at the centrosome which is required for initiating mitotic entry. Ubiquitination mediated by CUL3-KLHL18 ligase does not lead to its degradation by the proteasome.

SUBCELLULAR LOCATION

Cytoplasm, Cytoskeleton, Nucleus, Cell projection, Microtubule.

FUNCTION

Aurora A is a member of a family of mitotic serine/threonine kinases. It is implicated with important processes during mitosis and meiosis whose proper function is integral for healthy cell proliferation. Aurora A is activated by one or more phosphorylations and its activity peaks during the G2 phase to M phase transition in the cell cycle. Activation occurs via autophosphorylation, and while 14 sites are subject to this, only the threonine residue at position 295 is required for activity. Though autophosphorylation mediates activation, a number of other proteins influence activation, including the spindle assembly factor TPX2 and p53